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. 2015 Oct 17;4(12):951–960. doi: 10.1016/j.molmet.2015.09.013

Figure 1.

Figure 1

TRAF3 levels in the liver are aberrantly higher in obese mice. A. C57BL/6 male mice (8–9 weeks) were fasted for 24 h and then refed for 3 h. Liver TRAF3 mRNA abundance was measured by qPCR and normalized to β-actin expression. Randomly-fed: n = 8, fasted: n = 8, refed: n = 8. B. C57BL/6 male mice were fasted and refed as described above. Liver extracts were immunoblotted with antibodies against TRAF3 or tubulin. C. C57BL/6 male mice (7–8 weeks) were fed a chow diet or HFD for 10 weeks. WT and ob/ob mice (13 weeks) were fed a chow diet. Liver extracts were immunoblotted with antibodies against TRAF3 or tubulin. D. C57BL/6 male mice (10 weeks) were injected with STZ. Liver extracts were prepared 4 weeks after STZ treatments and immunoblotted with antibodies against TRAF3 or tubulin. E. Primary hepatocytes were prepared from C57BL/6 males (8–9 weeks) and grown overnight in serum-free DMEM supplemented with 0, 25 or 100 mM G-glucose. Cell extracts were immunoblotted with the indicated antibodies. *p < 0.05.