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. 2015 Oct 17;4(12):951–960. doi: 10.1016/j.molmet.2015.09.013

Figure 7.

Figure 7

Overexpression of recombinant TRAF3 in the liver induces insulin resistance and glucose intolerance in lean mice. C57BL/6 male mice (8–9 weeks) were infected with GFP or TRAF3 adenoviruses via tail vein injection. A. Body weight and overnight-fasted blood glucose and insulin levels 14 days after adenoviral infection. B–D. GTTs (glucose: 2 g/kg body weight), ITTs (insulin: 0.5 unit/kg), and PTTs (pyruvate: 2 g/kg) were performed 6–10 days after infection. E. Fourteen days after infection, mice were fasted for 20–24 h and stimulated with insulin (0.75 units/kg) for 5 min. Liver extracts were immunoblotted with antibodies against phospho-Akt (pThr308 and pSer473), Akt, or TRAF3. F. Phosphorylation of Akt was quantified and normalized to total Akt levels. *p < 0.05.