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. 2016 Jan 29;6:20267. doi: 10.1038/srep20267

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A–L) WISH of 30 hpf normal (A,C,E,G,I,K) and Six3-deficient (B,D,F,H,J,L) embryos. (A,B) vax1 expression. Brackets marks POAs, arrows point at OSs. (C–F) vax2 expression in the POA, OS and ventral retina. Brackets marks POAs, arrows point at OSs. t, telencephalon. (G,H) pax2a expression in the optic fissure (arrowheads in G,H) and OS (arrow in G, missing in H). (I,J) tbx5 expression in dorsal retina. White lines mark the width of the region where high tbx5 levels are observed. (K,L) raldh2 expression in the dorsal retina. Arrows mark limits of high raldh2 expression. (A–D) are ventral views, anterior is up. E-L are lateral views, anterior to the left, dorsal up. Scale bars are 50 μm.