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. 2016 Jan 29;6:20267. doi: 10.1038/srep20267

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Copyright © 2016, Macmillan Publishers Limited

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(A–H) Expression of signalling molecules in anterior diencephalic midline of normal (A,C,E,G) and Six3-deficient (B,D,F,H) 32 hpf embryos. (A–F) frontal views, (A’–F’) the same embryos as in (A–F) respectively, lateral views. (A,A’,B,B’) slit1a expression. Asterisk in (B) marks an apparently single commissure. (C,C’,D,D’) slit2 expression. Asterisk in (D) marks what appears as a single commissure. (E,E’,F,F’) slit3 expression. Asterisk in F marks the reduced POA. Brackets in all lateral views mark the POA flanked by AC and POC. (G,H) sema3d expression. Arrows point at the POA. AC, anterior commissure; POA, preoptic area; POC, post-optic commissure; t, telencephalon. (G,H) are dorsal views, anterior down. Anterior is to the left in lateral views. Scale bars are 50 μm.