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. 2016 Jan 29;6:20171. doi: 10.1038/srep20171

Figure 3. HFD induces MMP-12 expression in renal glomeruli.

Figure 3

(A) MMP-12 expression in apo E−/− and apo E−/−MMP-12−/− mice fed normal chow or HFD for 3, 6, and 9 months was detected by immunohistochemistry. Scale bar = 20 μm. (B) Quantitative analysis of MMP-12 expression calculated as a percentage of positive staining within the glomerular area (10 glomeruli per kidney per animal, n = 12 per group). (C) HFD-induced MMP-12 mRNA expression in isolated renal glomeruli, as evaluatedby qRT-PCR. Values represent means ± SEM (n = 6) in each group. *P < 0.05 vs. mice on normal chow; P < 0.05 vs. mice on HFD at the same time point.