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. 2016 Jan 29;6:20171. doi: 10.1038/srep20171

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(A) FN expression in apo E^−/− and apo E^−/−MMP-12^−/− mice fed normal chow or HFD for 3, 6, and 9 months was detected by immunohistochemistry. (B) Quantitative analysis of FN expression in glomeruli calculated as a percentage of positive staining within the glomerular area (10 glomeruli per kidney per animal, n = 12 per group). (C) HFD induced FN mRNA expression in isolated renal glomeruli evaluated by qRT-PCR. Values represent means ± SEM (n = = 6 per group). *P < 0.05 vs. mice on normal chow; ^†P < 0.05 vs. mice on HFD at the same time point.