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. 2016 Jan 29;6:18418. doi: 10.1038/srep18418

Figure 1. Purification of the protein subunits of the Mtb DNA pol III holoenzyme and reconstitution of leading-strand replication.

Figure 1

(a) Gene name, mass and predicted function of Mtb DNA Pol III subunits, and their percent identity to corresponding subunits in the E. coli Pol III system25. (b) Coomassie Blue-stained 12% SDS-polyacrylamide gel of the purified DNA pol III holoenzyme subunits. The positions of subunits in the gel are indicated by arrows. All purified proteins were verified by peptide mass fingerprinting (PMF) using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). (c) Reconstitution of leading-strand replication by the α subunit alone (left panel) and by the holoenzyme (right panel). The position of the fully extended M13mp18 DNA (TF II, 7249 bp) is indicated on the left. Size markers are shown on the right. Results presented are representative of at least three replicate experiments.