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. 2016 Jan 29;6:19699. doi: 10.1038/srep19699

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Recipient mice of the genotype indicated were injected i.v. with 2 × 10^e5 B16 melanoma cells. Macroscopic lung metastases (A) were counted (B). Lung cells were isolated and analysed by flow cytometry for frequencies of (D) NK cells, (E) KLRG1⁺ NK cells, (G) total CD8⁺ T cells and (H) KLRG1⁺ CD8⁺ T cells. Frequency in healthy lungs of total (C) NK cells and (F) CD8⁺ T cells are also shown. Statistics: ***p<=0.001; **p<=0.01; *p<=0.05; ns = non significant.