Figure 5. Crawling locomotion analysis of of C. elegans sarcomere mutants.

(a) Schematic diagram of crawling locomotion assays. The nematodes were put on fresh NGM plates solution and their head was gently touched by a platinum wire to induce the backward crawling locomotion. Three quantitative parameters were extracted from the threshold-segmented images: (b) frequency, (c) wavelength, and (d) amplitude of the crawling waves normalized by the length of the animals. n ≥ 20. ^*P < 0.01; ^**P < 0.001; ^***P < 0.0001.