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. 2016 Jan 29;6:19910. doi: 10.1038/srep19910

Figure 4. Function study of miR-193b-3p in preeclampsia.

Figure 4

We evaluated the efficiency of Pre-miR-193b-3p and miR-193b-3p inhibitor (A). Trophoblast (HTR-8/SVneo) cells were transiently transfected with pre-miR-193b-3p, miR-193b-3p inhibitor or the corresponding control constructs (negative control, NC). The migration ability was assessed 24 h from the scratch (B). To investigate the effect of miR-193b-3p overexpression, 54 scratch spots were assessed for either Pre-miR-193b-3p group or NC pre-miR-193b-3p group. To investigate the effect of miR-193b-3p inhibition, 10 scratch spots were assessed for each experiment group. The transwell migration assay was assessed 6 h from plating cells (C). The transwell invasion assay was assessed 24 h from plating cells (D). The number of invaded cells was counted in 5 different fields of each membrane under light microscope. All experiments were repeated three times. Data are shown as the mean ± SEM. Significant differences were determined by Student’s t test. ***P < 0.001, **P < 0.01.