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. 2016 Jan 29;7:20069. doi: 10.1038/srep20069

Figure 2. Tricellulin is a determinant for bacterial localization in tTJs.

Figure 2

(a) Caco-2 cells were transfected with tricellulin-targeted siRNA. A scrambled siRNA was utilized as a negative control. At 72 h after transfection, whole cell lysates were subjected to Western blot analysis using anti-tricellulin antibody. β-actin served as a loading control. (b) Tricellulin knockdown or control cells were infected with EGFP-expressing GAS strain at an MOI of 10 for 2 h in the presence of 2 μM human PLG. ZO-1 and tricellulin were immunostained and are shown as blue and red images, respectively. GAS-infected cells were analyzed using a confocal laser-microscope. Data shown are representatives of at least three separate experiments. (c) Bacterial localization in tTJs, bTJs, or other regions was assessed as described in the Methods section. Data obtained from ten fields of view (x630) are presented as the mean ± S.D. *P < 0.01.