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. 2016 Jan 29;7:20069. doi: 10.1038/srep20069

Figure 5. Bacterial translocation is mediated by interaction of SEN with human PLG.

Figure 5

(a) Caco-2 cells were grown using a Millicell filter system, and then infected with the NIH35 strain or sen mutants at an MOI of 10 for 2 h. After removing non-adherent bacteria, the ability of the GAS strains to translocate across epithelial cells at 8 h after infection in the presence of bovine PLG was assessed by examining medium samples obtained from the lower chambers. Data are shown as the mean ± S.D. of six wells from a representative experiment. *P < 0.01. (b) Human and bovine PLG were immobilized on a nitrocellulose membrane after serial two-fold dilutions. After blocking of the membrane, 40 μg of SEN was overlaid and the binding signal was detected with an anti-SEN antibody. (c) TRIC-EC1 protein was immobilized on microtiter plates, and increasing amounts of SEN were reacted in the presence of 1 μM human PLG or 1 μM bovine PLG. Bound SEN was detected using an anti-SEN antibody. Data are shown as the mean ± S.D. from three independent experiments. *P < 0.01. (d) The effect of human PLG on bacterial translocation was analyzed as described in (a). Data are shown as the mean ± S.D. of six wells from a representative experiment. *P < 0.01.