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. 2016 Jan 29;6:20059. doi: 10.1038/srep20059

Figure 7. miR-10a mediates the regulation of RA FLS proliferation, migration and invasion by TNF-α and IL-1β.

Figure 7

(A) RA FLS cells were transfected or treated as indicated, and BrdU was added to the cells, followed by incubation for 24 h. The incorporation of BrdU into newly synthesized cellular DNA was then detected with a POD-conjugated anti-BrdU antibody in a scanning spectrophotometer. Scramble miRNA was used as the negative control. (B) FLS cells were treated as indicated, and the cell scratch assay was performed to evaluate the migration ability of the cells. (C) Statistical analysis of (B). (D) FLS cells were transfected or treated as indicated, and a transwell assay was used to evaluate the effect of miR-10a on the invasion of the cells. (E) Statistical analysis of (D). nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group or between the indicated groups. M: miR-10a mimics, MS: mimics scramble, IS: inhibitor scramble. The data are representative of three experiments.