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Biotinylated kisspeptin-10 binding signals on TIDA neurons. (A) Incubation with biotinylated kisspeptin-10 yields an accumulation of signal (magenta) on the cell bodies (double-arrows) and fibers (single-arrow) of TIDA neurons (green). (B) Incubation with biotinylated kisspeptin-10 and excess non-labeled kisspeptin-10 reveals no binding signal. Bar=20 μm.
