Fig. 3. .

Biotinylated kisspeptin-10 binding signals on GnRH neurons. Signals in the median eminence (A–C), organum vasculosum of the lamina terminalis (D, E), and GnRH cell body in the medial preoptic area (F). Incubation with biotinylated kisspeptin-10 and excess non-labeled kisspeptin-10 yields no signal (C). The sections yield an accumulation of kisspeptin signals (magenta) on GnRH (green) fibers (A, B, D, E) but not on the cell body (F). Higher magnification views of (A) and (D) are shown in (B) and (E), respectively. Bars=50 μm (A, C, D); 20 μm (B, E, F).