Fig. 1.

Expression of cMyc plus shp53 in the liver induces well-differentiated HCC. a Schematic illustration of the experimental procedure to generate transgenic livers expressing cMyc plus GFP (control), and cMyc plus shp53. Hydrodynamic transfection was performed using a mixture of indicated plasmids. b Gross morphology (upper panels) of livers harvested from mice of each group at 7 months post hydrodynamic injection. Images of H&E staining in liver sections are shown below. Well-differentiated HCCs developed in cMyc plus shp53 mice, while the control mice did not develop tumors. Scale bar, 100 μm. c Protein expression levels of cMyc and p53 in liver of cMyc plus GFP mice (control) and liver tumor of cMyc plus shp53 mice. d Images of IHC staining for GFP and cMyc in liver sections of indicated groups. “T” denotes tumor and “N” denotes non-tumorous liver parenchyma tissue. Scale bar, 100 μm