Table 1.

Nucleotide sequences of primers and operating condition for PCR analysis

Gene		Nucleotide sequence	Operating condition
GAPDH	sense	5′-AACTTTGGCATTGTGGAAGG-3′	94 °C, 30s 58 °C, 30s 72 °C, 30s, 30 cycles
	antisense	5′-ACACATTGGGGGTAGGAACA-3′
IL-1β	sense	5′-GGCTGTGGAGAAGCTGTGGC-3′
	antisense	5′-GGGTGGGTGTGCCGTCTTTC-3′
TNF-α	sense	5′-GCAGAAGAGGCACTCCCCCA-3′
	antisense	5′-GATCCATGCCGTTGGCCAGG-3′
IL-4	sense	5′-TCAACCCCCAGCTAGTTGTC-3′
	antisense	5′-TGTTCTTCGTTGCTGTGAGG-3′

T thymine, A adenine, C cytosine, G guanine, GAPDH glyceraldehyde-3-phosphate dehydrogenase, IL interleukin, TNF tumor necrosis factor