Fig. 8.

NEAT1 regulates CDK6 through modulating miR-107. a TargetScan database predicated CDK6 as a target of miR-107. b miR-107 decreased luciferase activity of 3′UTR but not that of 3′UTR-NC and 3′UTR-MU. miR-NC indicated scramble miRNA used as the negative control for miR-107. **P < 0.01. c Starbase database predicted the target site between CDK6 and miR-107. d Real-time PCR showed that miR-107 level significantly increased after NEAT1 knockdown in Hep-2 cells. **P < 0.01. e Western blot analysis showed that CDK6 protein level decreased after NEAT1 knockdown in Hep-2 cells. Lane 1 and 2: control Hep-2 cells; Lane 3 and 4: Hep-2 cells transduced with NEAT1 siRNA. GAPDH was loading control. f Western blot analysis showed that CDK6 protein level decreased after NEAT1 knockdown in Hep-2 cells, but was restored after the knockdown of miR-107. GAPDH was loading control