Table 2.

mRNA expression levels in midguts of prunetin-treated male w¹¹¹⁸D.melanogaster compared to controls.

Target gene	Expression vs. control	±SEM	p-Value
α-Cat	1.001	0.067	0.992
arm	1.012	0.123	0.962
α-Spec	0.926	0.064	0.629
β-Spec	1.499	0.243	0.239
cora	0.935	0.013	0.666
Pax	0.982	0.079	0.927
pyd	0.845	0.179	0.480
shg	0.746	0.107	0.342

Male age-matched flies were fed a control diet (0.05% DMSO) or a prunetin supplemented diet (25 µM) for 10 days. mRNA expression levels were determined via two-step qRT-PCR and were normalized to the expression level of the housekeeping gene alpha-Tubulin at 84B. Values represent the mean±SEM of three independent experiments. n=3 (75 midguts per group in total, at least). Statistical evaluation: Student's t-test or Mann-Whitney-U.