Fig. 1.

E− embryos have morphological defects and increased mortality compared to E+ embryos. Representative pictures from the two diet groups at 120 hours post-fertilization (hpf). A. Uninflated swim bladders in elsewise morphologically normal E− embryos that survived to this stage. B. An E+ embryo provided for comparison. Morphological evaluations were made using the zebrafish acquisition and analysis program (ZAAP). Phenotypic differences in E− embryos became evident between 48 and 72 hpf, with most mortalities occurring by 72 hpf. By 120 hpf, 75–88% of E− embryos from each spawn were malformed or dead.