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. 2016 Jan 11;8:165–174. doi: 10.1016/j.redox.2016.01.004

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© 2016 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 3 — E− and E+ embryos display significantly different PL and lyso-PL composition profiles during development. Heatmaps of identified PL or lyso-PL are shown. Sample intensities were normalized against an internal standard (DT-PC 13:0/13:0) and then scaled with Pareto scaling. Figures were generated using Metabolanalyst software. Heatmap trends (high [red], low [blue]) indicate that the E− embryos contain, overall, greater quantities of lyso-PL species early in development (24 hpf) when compared to E+ embryos, but many of these species, notably those containing DHA (22:6), are markedly depleted by 120 hpf. PL trends are highly varied; however, E− embryos contain lesser quantities of PL species with DHA (22:6). A. PC, phosphatidylcholine; B. LPC, lysophosphatidylcholine; C. PE, phosphatidylethanolamine; D. PI, phosphatidylinositol and PS, phosphatidylserine E. LPE, lysophosphatidylethanolamine; LPI, lysophosphatidylinositol and LPS, lysophosphatidylserine.