Fig. 5.

E− embryos show enhanced depletion of many lyso-PL species during development when compared with E+ embryos. Data were generated and analyzed as described in Fig. 4. Notably, the lyso-PL species showing the greatest difference between the E− and E+ conditions all contained DHA (means±SEM). Two-way ANOVA, Bonferroni's post-test for multiple comparisons (*p<0.05, **p<0.01, ***p<0.001, ****p<0.0001). LPC 16:0 and LPC 20:3 levels at 72 hpf were significantly altered in E− vs. E+ embryos when analyzed using unpaired t-tests and Sidak-Bonferroni post-test (p<0.001, higher in E−; and p=0.0176, lower in E−, respectively), as were LPI 20:4 levels at 120 hpf (p<0.0001, lower in E−).