Fig. 7.

H₂¹⁸O incorporation into PCs and lyso-PCs is greater in E− than in E+ embryos. A and C. Ratios of [M+2+H]⁺/[M+H]⁺ from H₂O-incubated E− vs. E+ embryos were similar (solid bars; no significant differences) in PCs (A) and lyso-PCs (C); ratios comparing label incorporation in H₂¹⁸O-incubated E− vs. E+ embryo (patterned bars) revealed enhanced labeling in E− embryos of DHA-containing PCs, as well as in LPC 16:0 and 22:6, suggesting increased PL remodeling. PC species (means±SEM) were identified and confirmed as described in Fig. 4; PC 38:6 (16:0/22:6); PC 40:6 (18:0/22:6); PC 40:8 (18:2/22:6); PC 42:7 (20:3/22:4). All PLs and lyso-PLs showed significant (p<0.001) label incorporation in H₂O- vs. H₂¹⁸O-incubated embryos within the same diet condition, with the exception of E+ embryos LPC 22:6 in H₂O- vs. H₂¹⁸O-incubated (no significant difference, indicating no significant remodeling in E+ embryos). Asterisks indicate statistical differences between H₂¹⁸O E− and E+ groups (red and blue patterned bars, respectively). B and D. [M+2+H]⁺/[M+H]⁺ in H₂¹⁸O minus [M+2+H]⁺/[M+H]⁺ in H₂O (mean±SEM) in E− vs. E+ embryos for PCs (B) and lyso-PCs (D). A–D: Two-way ANOVA with Tukey's (A,C) or Sidak's (B,D) post-test for multiple comparisons (*p<0.05, ** p<0.01, ***p<0.001, ****p<0.0001).