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. 2016 Jan 14;8:192–198. doi: 10.1016/j.redox.2016.01.008

Fig. 5.

Fig. 5

Activation of CBS by SAM. (A) Fold activation after HCT116 transfection with expression vectors containing either the naked mole-rat CDS with leucine, cysteine and serine at position 412 (native L412, L412C and L412S, respectively) or the human CDS with cysteine, leucine and serine at position 431 (native C431, C431L and C431S, respectively). (B) Fold activation after HCT116 transfection with expression vectors coding for chimeric CBS: naked mole-rat N-terminal heme binding and catalytic domain, and human regulatory domain (Hgl core and Hsa reg) and vice versa (Hsa core and Hgl reg). (C) Fold activation of CBS from different species. Hgl – H. glaber, Fmi-F. micklemi, Fme – F. mechowii, Mmu – M. musculus, Hsa – H. sapiens. In (A) (B) (C), data represent mean±SD of n biological replicates, p-value: *<0.05, **<0.01, ***<0.001 (one-way ANOVA followed by Tukey test). Data were normalized to the lysate from mock transfected cells.