Table 2.

Primer sequences used for RT-PCR analysis, site-directed mutagenesis, and cloning.

Gene	Primer names and sequences (5′-3′)
CBS	cbs_qPCRhum_1F: ATGCTGATCGCGCAAGAG; cbs_qPCRhum_1R: TCGCTCAGGAACTTGGTCAT
GAPDH	hs_GAPDH_1F: AACGGGAAGCTTGTCATCAATGGAAA; hs_GAPDH_1R: GCATCAGCAGAGGGGGCAGAG
Mmu CBS	mcbscDNA_EcoRI_1F: TCTAGGAATTCTCGACCCATCCTTGCTGAGTTTGT; mcbscDNA_PmeI_1R: CTAATGTTTAAACTCGATTGGGTGAGGAAGCTGGTAG
Hgl CBS CT1234TG	nmrCBSMut_1F: CCCACCGTCACCTGCGAGCACACCATC; nmrCBSMut_1R: GATGGTGTGCTCGCAGGTGACGGTGGG
Hgl CBS CT1234TC	nmrCBS_L412S_f: TGCTGCCCACCGTCACCTCCGAGCACACCATCGCCAT; nmrCBS_L412S_r: ATGGCGATGGTGTGCTCGGAGGTGACGGTGGGCAGCA
Fme CBS T355C T358G	CBSmechToAnsel_2F: GAGGACGCAGAGCGCGCCGGGATCCT; CBSmechToAnsel_2R: AGGATCCCGGCGCGCTCTGCGTCCTC

Hgl – H. glaber, Fme – F. mechowii, Mmu – M. musculus.