Fig. 4.

DPP III translocates from the cytosol into the nucleus in response to both hyperoxia and E₂ administration in hepatic tissue of female CBA/H mice. Representative confocal images, of both FITC (green) and DAPI (blue) channels, of each sample are shown. In sham-operated group of female CBA/H mice only very weak nuclear staining of DPP III can be observed (A); enhanced intensities of DPP III nuclear signal are found in the nucleus after hyperoxia (B), and after hyperoxia combined with ovariectomy (C); while the most intense nuclear signal was seen after combined treatments of hyperoxia and E₂ addition (p=0.042, sham vs. hovxe). (D). Graphical display of the ratio between average fluorescence intensities in the nucleus and the cytoplasm, normalized to the ratio of sham group. (E). Differences in signal intensities between test groups were further analyzed using post-hoc Wilcoxon signed-rank test (matrix of probabilities for the pairwise comparisons) (F). The number of samples was n=2 per each group. Results are expressed as mean±S.D. and normalized to sham group. Bars represent 5 μm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)