FIGURE 10.

Knockdown of B-Raf(V600E) or PAR-1 reduced melanoma TEM under shear conditions. A, Lu1205; B; UACC903 TEMs were measured at a shear stress of 2 or 4 dynes/cm² in the presence or absence of PFP. C and D, scrambled siRNA or siB-Raf(V600E)-transfected Lu1205 (C) and UACC903 (D) TEMs were measured at a shear stress of 2 or 4 dynes/cm² in the presence of PFP. E and F, transmigrations of Lu1205 (E) or UACC903 (F) across scrambled siRNA or siPAR-1-transfected HUVECs were measured at a shear stress of 2 or 4 dynes/cm² in the presence of PFP. Values are mean ± S.E. from three independent experiments. **, p < 0.01 compared with control.