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. 2015 Nov 23;291(5):2107–2118. doi: 10.1074/jbc.M115.693580

FIGURE 1.

FIGURE 1.

O-GlcNAc maintained glucose-responsive insulin secretion in Min6 cells. A and B, nuclear enrichment from 1-h pharmacologically treated Min6 cells was immunoblotted with the O-GlcNAc monoclonal antibody Mab10. α-Tubulin served as the loading control (n = 6.). C, insulin secretion levels after 1 h of pharmacological stimulation from Min6 cells, quantified using RIA. Values are presented as insulin secretion (pg/ml) over time (n = 6). D, 1-h and 3-h insulin secretion in response to LG, HG, and HG+GNS treatments in the media from Min6 cells quantified using RIA. Values are presented as insulin secretion (pg/ml) over time (n = 3). All data are shown as the mean ± S.E. Significance was determined using an ordinary one-way ANOVA (B and C) or an ordinary two-way ANOVA (D). *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 versus LG; ##, p < 0.01 versus HG. NS, not significant.