TABLE 3.

Fe³⁺-heme dissociation rate constants for FLRev-erbβ_HGS, H568F, and H568F/C384A

Rate constants are derived from double exponential fits of the raw data (see Fig. 6).

Protein	koff-fast; ΔAbsorbancea	koff-slow; ΔAbsorbancea
	s−1; % of total	s−1; % of total
FLRev-erbβHGS	(1.5 ± 0.4) × 10−4; 18 ± 3	(3.9 ± 0.8) × 10−6; 82 ± 3
FLRev-erbβ H568F	(2.1 ± 0.4) × 10−2; 64 ± 9	(5 ± 1) × 10−3; 36 ± 9
FLRev-erbβ H568F/C384A	(4.3 ± 0.3) × 10−2; 88 ± 1	(2.4 ± 1.0) × 10−3; 12 ± 1

^a Rate constants and ΔAbsorbance % values represent the average ± S.D. of three replicate experiments.