FIGURE 2.

Loss of PRMT1 in the CNS results in dynamic change in methyl arginine levels in the brain. A, ADMA, SDMA, and MMA levels in the brain of CTRL and CKO mice were quantitated by LC-MS/MS at P10. Data were obtained from acid hydrolysate of proteins from each brain sample. The data shown are mean ± S.E. and were analyzed by a two-tailed Student's t test. n = 4 per each genotype.*, p < 0.05, **, p < 0.01. B, ADMA, SDMA, and MMA levels in the whole brains were detected by Western blot (upper panels) at P10, and loaded protein samples on membrane were confirmed to be equal by Coomassie Brilliant Blue (CBB) stain (lower panels). Molecular weight (MW) markers are indicated in kDa on the right of each panel.