FIGURE 5.

Inhibition of IgE antibody binding to rBla g 2 by the mutants. ELISAs were performed using three individual sera (a–c) and a sera pool (n = 6) (d) of Bla g 2-sensitized cockroach-allergic patients. Plots for sera 1 and 3 were very similar for the same mutants and at a range 0.1–100 μg/ml of inhibitor concentrations (shown only for sera 1). Therefore, the plot for serum 3 shows an additional experiment using only the single mutants at a lower inhibitor concentration range (0.0001–0.1 μg/ml). Inset in a shows inhibition curves from an additional experiment comparing the multiple mutants KK and KKF to rBla g 2.