TABLE 2.
Bla g 2 mutants and strategies for site-directed mutagenesis
| Mutant name | Substitutions | SMa | Purpose of the mutation |
|---|---|---|---|
| Double mutant (KK) | K132A/K251A | 1 | Epitopes for mAb 7C11 and 4C3 that interfere with IgE antibody binding |
| Triple mutant (KKF) | K132A/K251A/F162Y | 1, 2 | Epitopes for mAb 7C11 and 4C3 and explore Phe on molecular surface |
| F162Y | F162Y | 2 | Explore Phe on molecular surface |
| F300Y | F300Y | 2 | Explore Phe on molecular surface |
| Mutant A | E240A/R242cA/K246A | 3 | Explore Bla g 2 symmetry by mutating site located at the opposite lobe from mAb 7C11 |
| Mutant B | K241A/R243A | 3 | Explore Bla g 2 symmetry by mutating site located at the opposite lobe from mAb 7C11 |
a SM indicates strategies for mutagenesis.