FIGURE 6.

Elevated CAS expression contributes to increased sensitivity to TRAIL in Myc-overexpressing cells. a, WT and Myc- MCF10A cells were treated with 5 ng/ml TRAIL for the indicated time and then harvested for Western blot analysis to detect caspase-3 activation. b, WT and Myc- MCF10A cells were treated with the indicated concentration of TRAIL for 8 h and assayed for cell viability. c and d, MCF10A-Myc cells transfected with NT or CAS siRNA were treated with TRAIL (1.25 ng/ml for 4 h) for measurement of caspase-3/7 activity (c) or for 8 h with the indicated concentrations for measurement of cell viability (d). Representative phase-contrast images displaying cell morphology and Western blots confirming knockdown of CAS are shown. All values are mean ± S.E. from three independent experiments. *, p < 0.05.