Skip to main content
. 2015 Dec 10;291(5):2485–2498. doi: 10.1074/jbc.M115.697037

FIGURE 4.

FIGURE 4.

Overexpression of ZapC(K89D) and ZapC(K94D) did not interfere with cell division distinct from WT ZapC. A, 10-fold serial dilutions of WT (MG1655) cells expressing vector alone (KHH216), WT ZapC (KHH210), or mutant ZapC (KHH211, KHH212, KHH213, and KHH214) were spotted on LB Spec media without or with IPTG and incubated overnight at 37 °C. B, 10-fold serial dilutions of WT cells expressing GFP fusion plasmid alone (KHH240), WT ZapC-GFP (KHH236), ZapC(K89D)-GFP (KHH237), or ZapC(K94D)-GFP (KHH239) were spotted on LB Amp agar without or with IPTG and incubated at 37 °C.