FIGURE 6.

The ZapC(K94D) mutant did not promote FtsZ polymeric assemblies in vitro. A, equivalent aliquots of supernatants (S) and pellets (P) from co-sedimentation reactions in the presence of GTP containing FtsZ with either WT ZapC or ZapC(K94D) were separated by SDS-PAGE and visualized. Lane 1, FtsZ alone; lanes 2 and 3, FtsZ with ZapC at 1 and 5 μm, respectively; lanes 4 and 5, FtsZ with ZapC(K94D) at 1 and 5 μm, respectively; lane 6, ZapC(K94D) alone. The bar graph shows average amounts with S.D. of FtsZ (gray) or ZapC or ZapC(K94D) (black) amounts that were recovered in the pellet in each reaction and was compiled from three independent trials. Band intensities were measured using Image J. B, negative-stained electron microscopy images of FtsZ polymer assemblies in the presence of GTP alone (i), with WT ZapC (ii), and with ZapC(K94D) (iii). Bar = 200 nm.