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. 2015 Oct 29;291(5):2499–2509. doi: 10.1074/jbc.M115.668236

FIGURE 2.

FIGURE 2.

CaMDN increases the open probability but not unitary conductance of the Kv7.4a channel. A and B, a set of single-channel traces was recorded from CHO cells expressing either hKv7.4a alone (gray) or hKv7.4a + CaMDN (black) at a −60 mV step potential. The dotted lines represent closed states. Representative amplitude histograms of hKv7.4a alone and hKv7.4a + CaMDN at −60 mV are shown on the right. c, closed; o, open; I, current. C, data from amplitude histograms of applied voltages were used to generate current-voltage relationships. The single-channel conductances were 11 ± 0.6 pS (n = 9) (hKv7.4a) and 12 ± 0.8 pS (n = 10) (hKv7.4a + CaMDN), respectively. D and E, the open probability of hKv7.4a and hKv7.4a + CaMDN. Mean open probabilities are indicated by the dotted lines. F, exponential fits to the first latency histograms are shown. Time constants of hKv7.4a and hKv7.4a + CaMDN are indicated in milliseconds, and their median values are 46 ± 6 and 87 ± 9 ms (n = 7), respectively.