Skip to main content
. 2016 Jan 15;129(2):269–276. doi: 10.1242/jcs.177675

Fig. 3.

Fig. 3.

Sp5 is a direct target of Stat3. (A) qRT-PCR analysis of Socs3 and Sp5 expression levels in 46C mESCs deprived of LIF overnight and stimulated with LIF for 1 h in the presence or absence of JAK inhibitor I (JAKi) or cycloheximide (CHX, 50 mg/ml). Data represent mean±s.d. of three biological replicates. **P<0.01 vesus 46C NT. NT, no treatment. (B,C) qRT-PCR analysis of Socs3 and Sp5 in GY118F and Stat3ER transfectants deprived of LIF overnight and stimulated with GCSF or 4-HT for 1 h. Data represent mean±s.d. of three biological replicates. **P<0.01 versus NT. (D–F) Western blot analysis of total and phosphorylated Stat3 and β-catenin in wild type, and GY118F- and Stat3ER-transfected mESCs. p, phosphorylated protein. (G,H) qRT-PCR analysis of Socs3 and Sp5 in β-catenin-null or 46C mESCs treated with LIF or CHIR for 2 h in the presence or absence of JAK inhibitor I. Data represent mean±s.d. of three biological replicates. *P<0.05, **P<0.01 versus NT.