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. 2016 Jan 15;129(2):269–276. doi: 10.1242/jcs.177675

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Forced expression of Sp5 promotes EpiSC reprogramming to naïve pluripotency. (A) Phase contrast and alkaline phosphatase (AP) staining images of 46C mESCs and 46C mESC-derived EpiSCs. Scale bar: 100 μm. (B) qRT-PCR analysis of expression patterns of Sp5, Oct4, Rex1 and Fgf5 in 46C mESCs and EpiSCs. Data represent mean±s.d. of three biological replicates. **P<0.01 versus 46C mESCs. (C) Western blot analysis for Sp5 in 46C EpiSCs transfected with vector expressing FLAG-tagged Sp5. (D) Phase contrast and alkaline phosphatase staining images of Sp5 EpiSC transfectants and Sp5 EpiSC transfectant-derived induced pluripotent stem cells (iPSCs). Scale bar: 100 μm. (E) qRT-PCR analysis of Oct4, Nanog, Stella, Nr0b1, Klf2, Rex1, Fgf5 and Sox17 expression in Sp5 EpiSCs and two Sp5 Epi-iPS colonies. Data represent mean±s.d. of three biological replicates. **P<0.01 versus Sp5 EpiSCs. C1 and C2 are the individual colonies of ESC-like cell. Epi-iPS, EpiSC-derived iPSCs.