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. 2016 Jan 1;129(1):228–242. doi: 10.1242/jcs.177741

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Longitudinal intensity profiling localises actin and aldolase towards the rear of the invading merozoite tight junction. Heatmaps, normalised intensity plots and single-slice images of example merozoites for the longitudinal intensity profiling of mRON4 (green) versus (A) rabbit actin (rAct; red) or (B) rabbit aldolase (rAldo; red) antibody labelling. The boxed regions in DIC images are shown magnified to the right. Arrows indicate the position of the RON4-labelled tight junction. White boxes denote approximate front and back position of merozoite as determined in the DIC channel. See also Fig. S1D,E. Scale bars: 1 μm.