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. 2016 Jan 1;129(1):228–242. doi: 10.1242/jcs.177741

Fig. 6.

Fig. 6.

MTRAP is cleaved irrespective of invasion or egress from the schizont. (A) Immunoblot analysis of a timecourse of tightly synchronised schizonts and early ring samples grown in the presence or absence of inhibitors of egress (E-64) or invasion [heparin (hep)]. Labelling with rM-tail antibodies labels full-length MTRAP (∼75 kDa) and cleavage products (∼38/44 kDa), including the tail stub (∼15 kDa). (B) Immunoblot analysis of tightly synchronised late-schizont- and early-ring-stage parasites grown in the presence or absence of inhibitors of egress [antipain and leupeptin (A+L)] or E-64. Labelling with rM-tsr antisera identifies full-length MTRAP (∼75 kDa) and the cleaved TSR domain (∼25 kDa). rM-tail labelling identified products as in A. (C,D) Immunofluorescence analysis of very late-stage schizonts (C) treated with E-64 to prevent egress or (D) from untreated culture, co-labelled with mAMA1 (1F9) monoclonal antibodies (green) and rM-tail (left), rM-mid (middle) or rM-tsr (right) antibodies (red in respective images). Scale bars: 1 μm.