Table 1:

Sample sizes (n) and mean values ± SEM for physiological variables of sockeye salmon 30 min after one of three simulated fisheries capture-and-release treatments [control (C), no assisted ventilation (N), and assisted ventilation (A)] and two temperatures (16 and 21°C)

Physiological variable	Fisheries capture treatment	16°C		21°C		Treatment		Temperature		Interaction
		n	Mean ± SEM	n	Mean ± SEM	F	P-value	F	P-value	F	P-value
Plasma lactate (mmol l−1)	C	18	1.65 ± 0.15a	15	2.62 ± 0.38c	250.1	<0.0001	0.9	0.3444	4.19	0.018
	N	18	13.38 ± 0.64b	16	11.73 ± 0.98d
	A	19	13.06 ± 0.78bd	16	14.11 ± 0.98b
Plasma cortisol (ng ml−1) in males	C	10	120.0 ± 19.6a	10	162.8 ± 29.3a	28.25	<0.0001	4.79	0.0335	4.71	0.0136
	N	10	387.5 ± 35.9b	8	268.3 ± 15.3c
	A	10	350.5 ± 26.7b	6	273.6 ± 34.4c
Plasma cortisol (ng ml−1) in females	C	8	301.0 ± 41.3x	5	339.8 ± 64.0	13.84	<0.0001	0.07	0.7885	0.64	0.533
	N	8	531.0 ± 59.7y	8	526.2 ± 57.1
	A	9	586.1 ± 32.5y	10	521.5 ± 24.1
Plasma glucose (mmol l−1)	C	18	6.10 ± 0.34x	15	7.06 ± 0.63	19.42	<0.0001	0.72	0.3988	0.51	0.6023
	N	18	9.33 ± 0.57y	16	9.15 ± 0.70
	A	19	9.70 ± 0.59y	16	10.08 ± 0.47
Plasma Na+ (mmol l−1)	C	18	155 ± 3	15	152 ± 5	1.22	0.2986	3.84	0.0531	0.27	0.7673
	N	18	159 ± 3	16	154 ± 3
	A	19	163 ± 3	16	154 ± 3
Plasma Cl−(mmol l−1)	C	18	131.3 ± 1.3	15	131.3 ± 1.8	2.07	0.1314	0.07	0.794	0.03	0.97
	N	18	134.1 ± 1.3	16	133.3 ± 2.3
	A	19	134.9 ± 2.1	16	134.5 ± 1.0
Plasma K+ (mmol l−1)	C	18	1.1 ± 0.1x	15	1.4 ± 0.2	17.36	<0.0001	11.77	0.0009	1.66	0.1955
	N	18	0.4 ± 0.04y	16	0.8 ± 0.1
	A	19	0.6 ± 0.1y	16	0.8 ± 0.1
Plasma osmolality (mosmol kg−1)	C	18	316 ± 2x	15	314 ± 3	33.7	<0.0001	0.15	0.6981	0.62	0.5388
	N	18	349 ± 3y	16	348 ± 3
	A	19	343 ± 6y	16	350 ± 4
Haematocrit (%)	C	18	41.1 ± 0.9x	15	41.7 ± 1.4	23.05	<0.0001	4.19	0.0434	1.35	0.2652
	N	18	44.6 ± 1.5y	16	49.9 ± 2.1
	A	19	50.0 ± 1.0z	16	52.0 ± 1.6
Haemoglobin (g l−1)	C	18	102 ± 2x	15	104 ± 3	4.56	0.0128	6.41	0.013	2.85	0.0628
	N	18	98 ± 3xy	16	112 ± 4
	A	19	110 ± 2y	16	112 ± 3
Mean corpuscular haemoglobin concentration (MCHC; g l−1)	C	18	249 ± 3x	15	252 ± 6	34.57	<0.0001	0.09	0.7641	0.74	0.4816
	N	18	221 ± 3y	16	226 ± 4
	A	19	222 ± 4y	16	217 ± 4

Two-way ANOVA results are presented for each physiological variable. The superscript letters present the results of Bonferroni multiple comparisons tests. Dissimilar lower-case letters denote significant differences between values, where a–d denote differences with respect to significant treatment × temperature interactions and x–z denote significant differences with respect to the treatment main effect across both temperatures.