Table 3.
Authorisation of feed additives reducing the mycotoxin content in feed.
| Feed additive | Regulation authorising the feed additive | Criteria included in the Regulation specifying the conditions of use | Method of analysis included in the Regulation to enforce the conditions of use of the authorisation |
| Microorganism strain DSM 11798 of the Coriobacteriaceae family | European Commission implementing Regulation (EU) No. 1016/2013 | Minimum content: 1.7 × 108 CFU kg–1 of complete feedingstuff with a moisture content of 12% and specific strain |
Enumeration of microorganism strain DSM 11798 of the Coriobacteriaceae: pour plate method using agar supplemented with oxyrase. Identification of microorganism strain DSM 11798 of the Coriobacteriaceae: pulsed-field gel electrophoresis (PFGE) |
| Bentonite | European Commission implementing Regulation (EU) No. 1060/2013 | Maximum content: 20 000 mg kg–1 feedingstuff with a moisture content of 12% Characterisation of active substance bentonite: ≥ 70% smectite (dioctahedral montmorillonite) < 10% opal and feldspar < 4% quartz and calcite AFB1-binding capacity (BC AFB1) > 90% |
For the determination of bentonite in feed additive: X-ray diffraction (XRD) For the determination of BC AFB1 of the additive: adsorption test carried out in a buffer solution at pH 5.0 with a concentration of 4 mg l–1 for AFB1 and 0.02% (w/v) for the feed additive |
| Fumonisin esterase EC 3.1.1.87 | European Commission implementing Regulation (EU) No. 1115/2014 | Preparation of fumonisin esterase produced by Komagataella pastoris DSM 26643 containing a minimum of 3000 U g–1 Minimum content: 15 U kg–1 of complete feedingstuff with a moisture content of 12% |
For the determination of fumonisin esterase activity: HPLC-MS/MS method based on the quantification of the tricarballylic acid released from the action of the enzyme on fumonisin B1 at pH 8.0 and 30°C |
Conditions of use and analytical methods are as specified in the corresponding European Union regulations authorising these feed additives. CFU, colony-forming unit; U, 1 U is the enzymatic activity that releases 1 μmol of tricarballylic acid per minute from 100 μΜ fumonisin B1 in 20 mM Tris-Cl buffer pH 8.0 with 0.1 mg ml–1 bovine serum albumin at 30°C. The purpose of the methods of analyses is to enforce the conditions of use. However, to enforce the maximum content of bentonite, no method of analysis suitable for official control could be identified.