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. Author manuscript; available in PMC: 2016 Dec 16.
Published in final edited form as: J Am Chem Soc. 2015 Dec 4;137(49):15558–15566. doi: 10.1021/jacs.5b10763

Figure 4.

Figure 4

Davies pulsed ENDOR spectra of 17Ow in X at the three canonical g-values, prepared with 17OH2 buffer: Y122F freeze quenched at 42 ms (black) and 4 s (red) after mixing; WT freeze-quenched at 42 ms (blue). As baseline a spectrum is included for abundance X(Y122F, green) (42 ms) prepared in natural-abundance buffer solution. Conditions: π pulse length = 80 ns, τ = 600 ns, repetition time = 50 ms, 34.971 GHz (Y122F), 34.93 GHz (WT), T = 2 K.