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. 2016 Jan 21;4:1279. Originally published 2015 Nov 17. [Version 2] doi: 10.12688/f1000research.7142.2

Figure 2. Expression of ZO-1 and AQP4 in 2D- and 3D-astrocyte cultures.

Figure 2.

Immunofluorescence staining in situ for expression of ZO-1 in hCMEC/D3 cells (top) and AQP4 in human primary astrocytes (bottom) in 3D gel co-cultures (left) compared with conventional 2D solo cultures on a hard surface (right). 2D and 3D cultures were prepared, cultured, stained and imaged under the same conditions.