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. 2016 Jan 21;4:1279. Originally published 2015 Nov 17. [Version 2] doi: 10.12688/f1000research.7142.2

Table 1. Antibodies.

Antibody* Usage Conc. Supplier
Aquaporin-4
   Rabbit
Primary, indirect IF 2μg/ml Santa Cruz
Biotechnology Inc.
#sc-20812
Claudin-5
   Mouse IgG
Primary, indirect IF 2.5μg/ml Invitrogen Ltd
#35-2500
EDNRB
   Rabbit
Primary, indirect IF 8μg/ml Alomone labs
#AER-002
ZO-1
   Rabbit
Primary, indirect IF 2.5μg/ml Zymed
#61-7300
P-gp
   Mouse IgG
Primary, indirect IF 5μg/ml Kamiya Biomedical Co.
#MC012
GFAP
   Mouse IgG
Primary, indirect IF 2.4μg/ml Sigma Aldrich Inc.
# G3893
S100β
   Rabbit
Primary, indirect IF 1/200 Abcam
#ab52642
TE7
   Mouse IgG
Primary, indirect IF 0.67μg/ml Millipore (UK) Ltd
#CBL271
O1
   Mouse IgM
Primary, indirect IF 1μg/ml R&D systems
#MAB1327
CD68
    Mouse IgG
Primary, indirect IF 2μg/ml Dako (UK) Ltd
#F7135
PECAM1
   Mouse IgG
Primary, indirect IF 10μg/ml R&D systems
#BBA7
PECAM1-PerCP-
eFluor710
Direct IF 1.25μg/ml eBioscience Ltd
#46-0319-42
Anti-mouse-IgG-
AlexaFluor 488
Secondary, indirect IF 10μg/ml Invitrogen Ltd
#A11001
Anti-mouse-IgG-
AlexaFluor 555
Secondary, Indirect IF 10μg/ml Invitrogen Ltd
#A21422
Anti-mouse-IgM Secondary, Indirect IF 5μg/ml AbD Serotec
#MCA199F
Anti-rabbit-IgG
AlexaFluor 488
Secondary, Indirect IF 10μg/ml Invitrogen Ltd
#A11008

* All mouse primary antibodies are monoclonal. All others are polyclonal.