Table 1. Antibodies.
Antibody* | Usage | Conc. | Supplier |
---|---|---|---|
Aquaporin-4
Rabbit |
Primary, indirect IF | 2μg/ml | Santa Cruz
Biotechnology Inc. #sc-20812 |
Claudin-5
Mouse IgG |
Primary, indirect IF | 2.5μg/ml | Invitrogen Ltd
#35-2500 |
EDNRB
Rabbit |
Primary, indirect IF | 8μg/ml | Alomone labs
#AER-002 |
ZO-1
Rabbit |
Primary, indirect IF | 2.5μg/ml | Zymed
#61-7300 |
P-gp
Mouse IgG |
Primary, indirect IF | 5μg/ml | Kamiya Biomedical Co.
#MC012 |
GFAP
Mouse IgG |
Primary, indirect IF | 2.4μg/ml | Sigma Aldrich Inc.
# G3893 |
S100β
Rabbit |
Primary, indirect IF | 1/200 | Abcam
#ab52642 |
TE7
Mouse IgG |
Primary, indirect IF | 0.67μg/ml | Millipore (UK) Ltd
#CBL271 |
O1
Mouse IgM |
Primary, indirect IF | 1μg/ml | R&D systems
#MAB1327 |
CD68
Mouse IgG |
Primary, indirect IF | 2μg/ml | Dako (UK) Ltd
#F7135 |
PECAM1
Mouse IgG |
Primary, indirect IF | 10μg/ml | R&D systems
#BBA7 |
PECAM1-PerCP-
eFluor710 |
Direct IF | 1.25μg/ml | eBioscience Ltd
#46-0319-42 |
Anti-mouse-IgG-
AlexaFluor 488 |
Secondary, indirect IF | 10μg/ml | Invitrogen Ltd
#A11001 |
Anti-mouse-IgG-
AlexaFluor 555 |
Secondary, Indirect IF | 10μg/ml | Invitrogen Ltd
#A21422 |
Anti-mouse-IgM | Secondary, Indirect IF | 5μg/ml | AbD Serotec
#MCA199F |
Anti-rabbit-IgG
AlexaFluor 488 |
Secondary, Indirect IF | 10μg/ml | Invitrogen Ltd
#A11008 |
* All mouse primary antibodies are monoclonal. All others are polyclonal.