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. Author manuscript; available in PMC: 2016 Jan 29.
Published in final edited form as: Cell Rep. 2016 Jan 7;14(3):572–585. doi: 10.1016/j.celrep.2015.12.056

Figure 2. Extracellular flux analysis demonstrates the bioenergetic deficiency of ΔwhiB3.

Figure 2

(A) Diagram of the probe of an XF96 cartridge and a well of the cell culture microplate. During the assay, ECAR is measured as an indication of H+ production from carbon catabolism and OCR as an indication of OXPHOS. (B) OCR profiles of Wt Mtb H37Rv (blue), ΔwhiB3 (red), ΔwhiB3:comp (green) in the presence of pyruvate (50 μM, M1) and after addition of two aliquots of CCCP to a final concentration of 1 μM and 8 μM (M7) indicated by the vertical dotted lines. (C) Phenogram of all three strains at M1 (after pyruvate addition) and M7 (after the second CCCP addition) demonstrating increased OCR and ECAR of Wt Mtb H37Rv and ΔwhiB3:comp after CCCP addition in contrast to the inability of ΔwhiB3 to respond to the uncoupling stress.