Fig. 5.
MPLA adjuvant increased the IgG responses of both trivalent and mixed vaccines, but reduced their differences. Mice (n = 6) were immunized with the two vaccines with MPLA adjuvant, respectively, the IgG titers specific to the P domain antigens of astrovirus (AstV), hepatitis E virus (HEV), and norovirus (NoV), respectively, were determined and compared. Phosphate buffered saline (PBS) was administered to mice as negative control. The resulting IgG titers specific to the P domain antigen of AstV (A), HEV (B), and NoV (C) were shown. The differences of the IgG titers between the two vaccines are indicated in folds, while their statistical differences are shown by star symbols (* P < 0.05, ** P < 0.01).