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. Author manuscript; available in PMC: 2017 Feb 10.
Published in final edited form as: Vaccine. 2016 Jan 9;34(7):905–913. doi: 10.1016/j.vaccine.2015.12.068

Table 1.

Primers that were used to generate expression constructs of recombinant proteins.

Construct Name Sequence (5′ to3′)a Sense Enzyme Note
pQE30-AstV P-12G-HEV P P2083 GCACGGATCCTCTATCTACCTGCCGCTGC + BamHI AstV P domain
P2104 ATATCGTCTCCTCCGCCTCCGCCTCCGCCTCCGCCTCCGCCTCCGCCGAACTGAACGGTACG − BsmBI AstV P domain (contains sequences for 12 Glycines)
P2062 TATTCGTCTCCCGGATCTCCGGCTCCATCTCGTCCGTTCTCTGTTC + BsmBI HEV P domain
P2105 GCACAAGCTTTTACGGGTAGTCAACGGTGTCTTC − HindIII HEV P domain
pQE30-AstV P-12G-HEV P-12G-NoV P P2142 ATATCGTCTCCCGCCGCAAAAGCAATCGCCACGGCAATCGCACG − BsmBI HEV P domain
P2143 TATTCGTCTCCGGCGGAGGCGGAGGCGGAGGCGGAGGCGGAGGCGGATCAAGAACTAAACCATTC + BsmBI NoV P domain (contains sequences for 12 Glycines)
P2144 ATATGTCGACTTACCCCGCTCCATTTCCCATGGGGGCAAGTGTGTAGAACTG − SalI NoV P domain
a

enzyme sites are underlined