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. Author manuscript; available in PMC: 2016 Sep 15.
Published in final edited form as: J Neuroimmunol. 2015 Jul 26;286:86–92. doi: 10.1016/j.jneuroim.2015.07.007

Figure 2.

Figure 2

Characterization of the dendritic surface antigen recognized by positive OMAS samples. Serum was incubated on live rat cortical cultures. Following fixation, fluorescent immunostaining was performed using primary antibodies against presynaptic (synaptophysin) or postsynaptic (PSD-95) elements, and human IgG labelled with Alexa 488 conjugated secondary antibodies. (A) Serum from a patient with AMPA-R encephalitis labels puncta (green) that colocalize with PSD-95 (red). (A′) Higher magnification view of boxed area in (A), followed by grayscale images of this boxed area as indicated. (B) Serum from a patient with AMPA-R encephalitis labels puncta (green) that are adjacent to, but do not colocalize with, synaptophysin (red). (B′) Higher magnification view of boxed area in (B), followed by grayscale images of this boxed area as indicated. (C) Positive OMAS serum labels puncta (green) that do not colocalize with PSD-95 (red). (C′) Higher magnification view of boxed area in (C), followed by grayscale images of this boxed area as indicated. (D) Positive OMAS serum labels puncta (green) that do not colocalize with synaptophysin (red). (D′) Higher magnification view of boxed area in (D), followed by grayscale images of this boxed area as indicated. Scale bars = 10 μm.