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. 2016 Jan 29;11(1):e0147764. doi: 10.1371/journal.pone.0147764

Fig 2. Immunofluorescence characterization and real-time quantitative PCR validation of primary cultured human NP cells.

Fig 2

(A) Fluorescence microscopy images showing collagenase type II was observed in NP cells. Nuclei were stained with DAPI, shown in blue. Images were acquired using laser scanning confocal microscopy under a 40×objective. (B) Fluorescence microscopy images showing aggrecan was observed in NP cells. Nuclei were stained with DAPI, shown in blue. Images were acquired using laser scanning confocal microscopy under a 40×objective. (C) Real-time RT-PCR analysis of negative NP cell marker gene collagen type I in NP cells, NP and AF. Real-time RT-PCR analysis was performed in triplicate and the expression levels of collagenase type I mRNAs were normalized to GAPDH mRNAs. (D) Real-time RT-PCR analysis of NP cell marker gene collagen type II in NP cells, NP and AF. Real-time RT-PCR analysis was performed in triplicate and the expression levels of collagenase type II mRNAs were normalized to GAPDH mRNAs. Error bars represent SD. As compared with control, ** indicates p<0.01.